Fig. 6: Influence of persistent GALNTL5 protein expression on cell structure.

A Fluorescence image of GALNTL5-GFP/GC-2spd cells treated with anti-calnexin antibody and without cumate induction. B Fluorescence image of GALNTL5-GFP/GC-2spd cells treated with anti-calnexin antibody after 2 days in the presence of cumate solution. In the cell marked with the white arrowhead, the ER is stained with anti-calnexin antibody and it extends widely throughout the cytoplasm. In the cell marked with the white arrow, the ER is enlarged and has a rough mesh structure. The cells marked with white asterisks show constriction of nuclei, and calnexin and GALNTL5 signals are seen around the nuclei. C Fluorescent image of GALNTL5-GFP/GC-2spd cells with anti-GM130 antibodies staining, and without cumate treatment. D Fluorescent image of GALNTL5-GFP/GC-2spd cells with anti-GM130 antibody after 2 days in the presence of cumate solution. In the cells marked with white asterisks, GM130 signals have accumulated on the nuclear membrane. In the cell with a smaller nucleus (white arrow), GM130 signals are barely visible. In each merged image, the nucleus was stained with DAPI. White scale bars, 20 µm.