Fig. 2: Validate CADD predicted compounds by biochemistry assays.
From: Developing novel Lin28 inhibitors by computer aided drug design
A Establish optimal FP assays by titrating the Lin28b ZKD protein (left), fluorescent labeled pre-let-7 RNA probe (middle), and non-fluorescent labeled pre-let-7 RNA probe. B FP assays screened all 32 compounds designed by CADD. C Establish optimized conditions for EMSA assays using Lin28b ZKD and fluorescent labeled pre-let-7 RNA probe. D increasing doses of 13 Lin28b inhibitors identified by FP assays were used in the optimized EMSA assays. Three independent biological replicates were performed for all the assays. All results are presented as the mean ± SD. Only one representative EMSA image was shown for each compound.
