Fig. 4: Ln268 strongly blocks cancer cell stemness of t-NEPC cells.
From: Developing novel Lin28 inhibitors by computer aided drug design
A, B DuNE cells were treated with C1632, Ln15, and Ln268 for 72 h. Selected Let-7 targeted genes, stem cell biomarkers and NE biomarkers were measured by real-time qPCR (A). Lin28b and its downstream HMGA2 protein levels were measured by immunoblotting (B). C DuNE spheroids were treated with 0–100 μM of C1632, Ln15, and Ln268 for 0–6 days. Spheroid sizes were measured and plotted. D DuNE cells were used to perform colony formation assays in the presence of C1632, Ln15 and Ln268 for 14 days. The number of colonies formed in each treatment counted and plotted. E DuNE cells under 3D culture conditions were treated with C1632, Ln15, and Ln268 at the indicated concentrations. Cell growth rates were measured by Incucyte. F DuNE cells were treated with 0–20 μM Ln268 for 144 h. FACS measured cell populations at each stage of cell cycling. Three independent biological replicates were performed for all the assays. All results are presented as the mean ± SD.
