Fig. 6: The VAMP3/LC3C-mediated autophagy pathway regulates c-Met recycling. | Cell Death Discovery

Fig. 6: The VAMP3/LC3C-mediated autophagy pathway regulates c-Met recycling.

From: Helicobacter pylori reduces METTL14-mediated VAMP3 m6A modification and promotes the development of gastric cancer by regulating LC3C-mediated c-Met recycling

Fig. 6: The VAMP3/LC3C-mediated autophagy pathway regulates c-Met recycling.The alternative text for this image may have been generated using AI.

a Western blotting was performed to detect c-Met expression in AGS cells treated with 3-MA or EBSS. b Western blotting was performed to detect c-Met expression in AGS cells transfected with the VAMP3 plasmid treated with 3-MA or the control for 24 h. c Coimmunoprecipitation (Co-IP) of the binding between exogenous VAMP3 and exogenous LC3C or LC3B in AGS cells cotransfected with Flag-VAMP3 plasmid and Myc-LC3C plasmid or Myc-LC3B plasmid. d Co-IP of endogenous c-Met and LC3C in AGS cells with VAMP3 knockdown. e Co-IP of endogenous c-Met and LC3C in AGS cells overexpressing VAMP3. f, g c-Met expression level was measured by Western blot in AGS and HGC27 cells with VAMP3 knockdown or overexpression. h Western blotting was performed to detect METTL14, VAMP3 and c-Met expression levels in AGS cells treated with Hp26695 at MOI = 100 for 12 h or wild-type METTL14 plasmid. *p < 0.05, **p < 0.01, ***p < 0.001.

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