Fig. 2: Ovarian transplantation of Exoc1-G-cKO ovaries into ROSAGRR/GRR ovaries. | Cell Death Discovery

Fig. 2: Ovarian transplantation of Exoc1-G-cKO ovaries into ROSAGRR/GRR ovaries.

From: Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice

Fig. 2: Ovarian transplantation of Exoc1-G-cKO ovaries into ROSAGRR/GRR ovaries.

Images of harvested ovaries nine weeks after ovary transplantation from three-week-old Exoc1-G-cKO (Exoc1flox/flox::Gdf9+/Cre) and Exoc1-G-ctrl (Exoc1+/flox::Gdf9+/Cre) mice into three-week-old ROSAGRR/GRR mice. A Representative immunofluorescence images for green fluorescence protein and Haematoxylin and Eosin staining. Dashed circles: transplanted donor ovarian regions a, c: Oocytes in donor ovaries. b, d: Oocytes in recipient ovaries. Scale bar = 500 µm (at low magnification), scale bar = 50 µm (high magnification). B Follicle growth in donor ovaries. Primordial and primary follicles, but not secondary follicles, were found in the Exoc1-G-cKO donor ovaries. C Comparison of transplanted and non-transplanted Exoc1-G-cKO mice ovaries. The number of primordial oocytes was significantly higher in transplanted Exoc1-G-cKO ovaries than in non-transplanted Exoc1-G-cKO mice ovaries (9 + 3 and 12 weeks). Three ovarian regions per mouse were measured, and the number of primordial oocytes in nine regions, 3 regions ×3 mice, was plotted. One-way analysis of variance.

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