Fig. 3: Cdk14 ablation inhibits the recovery from LPS-induced lung injury. | Cell Death Discovery

Fig. 3: Cdk14 ablation inhibits the recovery from LPS-induced lung injury.

From: CDK14 regulates the development and repair of lung

Fig. 3

A Representative histological morphology of hematoxylin-eosin and sirius red staining of lung from Cdk14 Ctrl and Cdk14 KO mice following LPS-induced lung injury. B Representative projected confocal images showing RAGE+ (green), SFTPC+ (orange) and EdU+ (red) cells in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about covered area of RAGE, SFTPC labelled AT2 cell number and EdU+ cell number in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. All data are normalized to Cdk14 Ctrl. Cdk14 Ctrl = 3 ~ 5；Cdk14 KO = 3 ~ 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. C Representative projected confocal images showing EMCN+ (green) blood vessels and NKX2.1+ (orange) lung epithelial progenitor cell and αSMA (red) labelled myofibroblasts in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about covered area of EMCN and αSMA and NKX2.1 labelled lung epithelial progenitor cell number in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. All data are normalized to Cdk14 Ctrl. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. D Diagram depicting for CD45+ cells in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about the number of CD45+ cells in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. E Diagram depicting for myeloid cells (Gr-1+, CD11b+) in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about the number of myeloid cells in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. F Diagram depicting for neutrophils (Ly6G+, CD11b+) in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about the number of neutrophils in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. G Diagram depicting for interstitial macrophages (CD64+) and monocytes (CD64-) in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Quantification about the number of interstitial macrophages and monocytes in the two groups. Cell frequencies show the percentage of target population in total cells. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. H Diagram depicting for B cells (B220+, CD19+) in LPS-treated Cdk14 Ctrl and Cdk14 KO mice lung. Cell frequencies show the percentage of target population in total cells. Quantification about the number of B cells in the two groups. Cdk14 Ctrl = 5；Cdk14 KO = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test.

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