Fig. 4: Cdk14 influences the proliferation and migration of endothelial and epithelial cells in vitro.

A RT-PCR showing the intracellular mRNA levels of Cdk14 in HUVECs and A549 cells treated with shCDK14. All data are normalized to shCtrl. shCtrl = 3；shCDK14 = 3. Error bars, mean \(\pm\) s.e.m. P values, t-test. B CCK-8 assays were performed to show the effect of shCDK14 on hampered proliferation in HUVECs or A549 cells. All data are normalized to shCtrl. shCtrl = 5 ~ 6；shCDK14 = 5 ~ 6. Error bars, mean \(\pm\) s.e.m. P values, t-test. C HUVECs were infected with shCDK14 to knockdown Cdk14. Flow cytometry analysis of HUVEC proliferative ability. All data are normalized to shCtrl. shCtrl = 8；shCDK14 = 8. Error bars, mean \(\pm\) s.e.m. P values, t-test. D A549 cells were infected with shCDK14 to knockdown Cdk14. Flow cytometry analysis of A549 cells proliferative ability. All data are normalized to shCtrl. shCtrl = 8；shCDK14 = 8. Error bars, mean \(\pm\) s.e.m. P values, t-test. E PI staining assays were performed to show the percentage of G2/M phase in HUVECs. All data are normalized to shCtrl. shCtrl = 4；shCDK14 = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. F PI staining assays were performed to show the percentage of G2/M phase in A549 cells. All data are normalized to shCtrl. shCtrl = 4；shCDK14 = 4. Error bars, mean \(\pm\) s.e.m. P values, t-test. G HUVECs were infected with shCDK14 to knockdown Cdk14, after 96 h of infection, transwell assays were performed, and pictures were taken 16 h later. All data are normalized to shCtrl. shCtrl = 9；shCDK14 = 9. Error bars, mean \(\pm\) s.e.m. P values, t-test. H A549 cells were infected with shCDK14 to knockdown Cdk14, after 96 h of infection, scratch assays were performed, and pictures were taken in 0 h and 48 h. All data are normalized to shCtrl. shCtrl = 6；shCDK14 = 6. Error bars, mean \(\pm\) s.e.m. P values, t-test.