Fig. 2: Forced expression of CRAT suppressed OC growth by inducing cell cycle arrest and apoptosis. | Cell Death Discovery

Fig. 2: Forced expression of CRAT suppressed OC growth by inducing cell cycle arrest and apoptosis.

From: CRAT downregulation promotes ovarian cancer progression by facilitating mitochondrial metabolism through decreasing the acetylation of PGC-1α

Fig. 2

A, B Overexpression of CRAT was determined by qRT-PCR and western blot analysis in two OC cell lines named HEY and SKOV3 (EV, empty vector; CRAT, expression vector encoding CRAT). C–E MTS cell viability C, colony formation D and EdU staining E assays were performed in HEY and SKOV3 cells with CRAT overexpressing. F The effect of CRAT overexpressing on cell cycle distribution was determined by flow cytometry analysis in HEY and SKOV3 cells. G The effect of CRAT overexpressing on cell apoptosis was determined by flow cytometry analysis in HEY and SKOV3 cells. Data presented as mean ± SEM of triplicate independent experiments, *P < 0.05.

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