Fig. 9: Decreased CRAT expression in OC cells is mainly caused by upregulation of miR-132-5p. | Cell Death Discovery

Fig. 9: Decreased CRAT expression in OC cells is mainly caused by upregulation of miR-132-5p.

From: CRAT downregulation promotes ovarian cancer progression by facilitating mitochondrial metabolism through decreasing the acetylation of PGC-1α

Fig. 9

A, B The mRNA and protein expressions of CRAT were determined by qRT-PCR and western blot assays in OC cells transfected with miR-132-5p. C miR-132-5p expression was analyzed by qRT-PCR assay in another 30-paired OC tissues and corresponding adjacent non-tumor tissues. D The relationship between miR-132-5p and CRAT expressions in tumor tissues from OC patients (n = 30). E Wild- and mutant-type CART 3’-UTR at the binding sites of miR-132-5p. F Luciferase assay was performed with CART 3’-UTR and CART 3’-UTR-mut reporters upon miR-132-5p transfection. G, J The effect of miR-132-5p transfection on CRAT downregulation-regulated in vitro OC growth G, H and metastasis I, J capabilities were evaluated. Data presented as mean ± SEM of triplicate independent experiments, *P < 0.05.

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