Fig. 4: diMe-Doxo is able to rescue mutant p63 transcriptional activity. | Cell Death Discovery

Fig. 4: diMe-Doxo is able to rescue mutant p63 transcriptional activity.

From: Anthracyclines disaggregate and restore mutant p63 function: a potential therapeutic approach for AEC syndrome

Fig. 4

A Molecular structures of diMe-Doxo. B BN-PAGE followed by p63 Western blot of primary keratinocyte lysates isolated from mice with the indicated genotypes. Cells were treated with 1 μM Epi, 1 μM Doxo or 0.5 μM diMe-Doxo for 36 h. After treatment, cells were harvested, lysed and analyzed for p63 levels. Samples were normalized for p63 amount by Western blot analysis. m monomer, d dimer, t tetramer. C–F Real-time RT-PCR analysis of the keratinocyte-specific p63 target genes Krt5, Krt14, Dsg1, and Dsp in primary keratinocytes following the treatment with the indicated compounds for 36 h. Data are represented as mean ± SD (WT n = 4; L514F/L514F n = 4). *p < 0.05, paired two-tailed t-test.

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