Fig. 3: METTL3 affected autophagy through targeting ATG7.

A, C, E mRNA levels of METTL3, ATGs (autophagy-related genes), and autophagy-related transcription factors in control and METTL3 knockdown cells under hypoxic conditions (n = 3). B, D, F mRNA levels of METTL3, ATGs (autophagy-related genes), and autophagy-related transcription factors in control and METTL3 overexpressing cells under hypoxic conditions (n = 3). G Western blot analysis of protein levels in H9c2 cells with METTL3 knockdown following hypoxia (n = 3). H Western blot analysis of protein levels in H9c2 cells with METTL3 overexpression following hypoxia (n = 3). I, K Western blot analysis of protein levels in H9c2 cells after transfection with or without METTL3 siRNA followed by treatment with or without ATG7 siRNA under the normoxic or hypoxic conditions (n = 3). M Cell viability was evaluated with the CCK8 assay (n = 5). O, P Immunofluorescence images of LC3 puncta in NRCMs treated in the same method as described above (n = 5). Scale bar: 10 μm. J, L Western blot analysis of protein levels in H9c2 cells after transfection with or without Ad-METTL3-OE followed by treatment with or without ATG7 overexpression plasmids under the normoxic or hypoxic conditions (n = 3). N Cell viability was evaluated with the CCK8 assay (n = 5). Q, R Immunofluorescence images of LC3 puncta in NRCMs treated in the same method as described above. Scale bar: 10 μm. All data were presented as mean ± SD. ns represents p > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.