Fig. 5: YTHDF2 mediated mRNA expression of ATG7 through m6A-dependent mechanism.
From: METTL3 regulates autophagy of hypoxia-induced cardiomyocytes by targeting ATG7
A mRNA levels of YTHDF1, YTHDF2, and YTHDF3 in control and YTHDF1 knockdown or YTHDF2 knockdown or YTHDF3 knockdown cells (n = 3). B, C Western blot analysis of protein levels in control and YTHDF1 knockdown or YTHDF2 knockdown or YTHDF3 knockdown cells for the indicated times (n = 3). D mRNA levels of YTHDF2 and ATG7 in control and YTHDF2 knockdown cells with or without hypoxia (n = 3). E Western blot analysis of protein levels in H9c2 cells after transfection with or without Ad-METTL3-OE followed by treatment with or without YTHDF2 siRNA under the normoxic or hypoxic conditions (n = 3). F RNA immunoprecipitation-qPCR (RIP-qPCR) analysis of the interaction of ATG7 with METTL3 in H9c2 cells transfected with or without YTHDF2 siRNA. Enrichment of ATG7 was measured by qPCR and normalized to input (n = 3). G H9c2 cells transfected with or without YTHDF2 siRNA were treated with 5 μg/ml actinomycin D for the indicated times (n = 3). All data were presented as mean ± SD. ns represents p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
