Fig. 3: YAP/TAZ modulates TRAIL-R2/caspase-8 apoptotic signaling upon ER stress. | Cell Death Discovery

Fig. 3: YAP/TAZ modulates TRAIL-R2/caspase-8 apoptotic signaling upon ER stress.

From: Regulation of ER stress-induced apoptotic and inflammatory responses via YAP/TAZ-mediated control of the TRAIL-R2/DR5 signaling pathway

Fig. 3

A A549 EGFP or A549 wtYAP cells were transfected with siRNAs against both YAP and TAZ (Y/T) as described in “Material and Methods”. A scrambled (SC) RNA was also used as non-targeting control oligonucleotide. After 6 h, cells were treated with doxycycline 1 µg/ml. Thirty hours post-transfection, thapsigargin 200 nM was added during 24 h. Cell death was measured as quantification of subG1 population. Caspase-8 (C8) activation and YAP knockdown were analyzed by western blotting. shScrambled (SC) or shcaspase-8 (C8) A549 cells (B) and shScrambled (SC) or shTRAIL-R2 (TR2) A549 cells (C) were transfected with SC or Y/T siRNAs. After 30 h, cells were treated with thapsigargin 200 nM during 24 h. and cell death was assessed by quantification of subG1 population. Protein knockdown was verified by immunoblot analysis. D shSC or shTR2 A549 cells were treated as in (C) and C8 activation was assessed by western blotting. Data represent mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, Two-way ANOVA with Tukey´s multicomparisons test.

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