Fig. 4: YAP/TAZ-TEAD signaling confers resistance to ER stress-induced apoptosis in tumor cells.

A A549 cells were cultured in the presence or absence of XAV939 5 μM during 24 h and transcriptional activity of TEAD was measured by luciferase assay with 8XGTII–lux reporter. B A540 cells were treated as in (A) and then thapsigargin 200 nM was added during 24 h. Cell death was analyzed by quantification of subG1 population. CHOP expression and caspase-8 processing were assessed by western blotting. C Microscope images showing TEADi-GFP expression in A549 cells after doxycycline treatment (1 μg/ml, 24 h) (scale bars 20 µm). D TEAD transcriptional activity measured by luciferase assay in TEADi-GFP A549 cells cultured in the presence or absence of doxycycline 1 μg/ml 24 h. E A549 TEADi-GFP cells were treated with doxycycline during 24 h before treatment with thapsigargin for 24 h. Cell death was measured as quantification of subG1 population. Data show the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, Unpaired T-test (A, D); Two-way ANOVA with Tukey´s multi comparisons test (B, E).