Table 1 Preparation of polyacrylamide hydrogels.
SOFT (0.5 kPa) | STIFF (144 kPa) | |
|---|---|---|
PBS 1× | 440 µl | 1975 µl |
ACRILAMYDE 40% | 50 µl | 150 µl |
BIS ACRYLAMIDE 2% | 7.5 µl | 150 µl |
APS 10% | 2.5 µl | 2.5 µl |
TEMED | 0.25 µl | 0.25 µl |
FINAL VOLUME | 500 µl | 500 µl |
SOFT (0.5 kPa) | STIFF (144 kPa) | |
|---|---|---|
PBS 1× | 440 µl | 1975 µl |
ACRILAMYDE 40% | 50 µl | 150 µl |
BIS ACRYLAMIDE 2% | 7.5 µl | 150 µl |
APS 10% | 2.5 µl | 2.5 µl |
TEMED | 0.25 µl | 0.25 µl |
FINAL VOLUME | 500 µl | 500 µl |
