Fig. 2: TMEM45A inhibits the cell cycle arrest, apoptosis and promotes proliferation in BRCA cells.

A Cell cycle analysis by Flow Cytometry showing the cell cycle distribution under 24 h of treatment with vehicle (DMSO) or 1 μM palbociclib and statistical analysis are shown below. B Cell apoptosis assay by Annexin-V/PI dual staining under 24 h of treatment with vehicle (DMSO) or 1 μM palbociclib and statistical analysis are shown on the right side. C The results of the colony formation assay under 14 days treatment with vehicle (DMSO) or 1 μM palbociclib and statistical analysis are shown on the right side. D 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay show the proliferative ability of BRCA cells under 24 h of treatment with vehicle (DMSO) or 1 μM palbociclib and statistical analysis are shown on the right side. *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significance.