Fig. 3: RNASeq analysis identifies the genes associated with the cell cycle. | Cell Death Discovery

Fig. 3: RNASeq analysis identifies the genes associated with the cell cycle.

From: CDK8/19 inhibition attenuates G1 arrest induced by BCR-ABL antagonists and accelerates death of chronic myelogenous leukemia cells

Fig. 3

The K562 cells were treated for 8 h with SenB, IM (1 μM each) and their combination. A A dot plot comparing the effects of IM vs untreated control and IM + SenB vs IM on the differentially expressed genes (DEGs) affected by IM (FC (fold change) >1.5, FDR (false discovery rate) <0.01) shows both SenB-sensitive (red dots) and -insensitive genes (blue dots). 6.1% (163 genes) were differentially expressed in IM and IM + SenB groups. B The heatmap of the effects of different treatments on the genes most strongly upregulated or downregulated by IM demonstrates predominant upregulation of IM-induced genes and downregulation of IM-inhibited genes by the addition of SenB. C Analysis of hallmark pathways (GSEA). Combined treatment upregulated the majority of pathways compared to IM alone but did not prevent the IM-induced changes in the majority of pathways. D Netplot of genes downregulated in IM + SenB cohort vs IM alone. IM + SenB downregulates cell cycle related genes (17.1% of all DEG downregulated by the combination vs IM alone).

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