Fig. 4: Combinations of IM and SenB affect cell cycle genes and proteins in K562 cells but not in KU812 counterparts. | Cell Death Discovery

Fig. 4: Combinations of IM and SenB affect cell cycle genes and proteins in K562 cells but not in KU812 counterparts.

From: CDK8/19 inhibition attenuates G1 arrest induced by BCR-ABL antagonists and accelerates death of chronic myelogenous leukemia cells

Fig. 4

K562 and KU812 cells were treated as indicated. Transcripts were analyzed by RNASeq, and proteins by immunoblotting. A SenB in combination with IM counteracts MYC and CDKN1B (p27Kip1) upregulation by IM. The abundance of MYC and CDKN1B mRNAs is shown as normalized counts per million (CPM) reads. False discovery rate (FDR) < 0.001. B SenB prevents IM-induced p27Kip1 accumulation. C Immunoblotting of c-Myc, p57Kip2, p18INC4c in K562 (left) and c-Myc, p27Kip1, p21Cip1, p18INC4c in KU812 (right) at the indicated time points. IM induces accumulation of CKIs and downregulation of c-Myc in K562 cells, SenB in combination with IM (I + S) partially reverses this effect, but not in KU812 cells. Β-actin is a loading control.

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