Fig. 4: Inhibition of autophagy in keratinocyte survival and proliferation following UVB exposure.
From: Loss of TC-PTP in keratinocytes leads to increased UVB-induced autophagy
A, B Effect of inhibition of autophagy on keratinocyte survival and proliferation in response to UVB irradiation. TC-PTP/Mock and TC-PTP/KO cells were pretreated with 3-MA (5 mM) for 1-h prior exposure to UV irradiation (10 mJ/cm2). Cells were then collected 16 h after UVB irradiation. A Immunoblot analysis of HaCaT TC-PTP/Mock and TC-PTP/KO cell lysates with antibodies specific for LC3, TC-PTP, and β-actin. B Cell viability of the cells was measured using WST-8 assay. *P < 0.005 by T-test for equality of means. C, D Effect of inhibition of autophagy on keratinocyte survival and proliferation in response to UVB irradiation. TC-PTP/Mock and TC-PTP/KO cells were pretreated with CQ (50 μM) for 1-h prior exposure to UV irradiation (10 mJ/cm2). Cells were then collected 16 h after UVB irradiation. C Immunoblot analysis of HaCaT TC-PTP/Mock and TC-PTP/KO cell lysates with antibodies specific for LC3, TC-PTP, and β-actin. D Cell viability of the cells was measured using WST-8 assay. *P < 0.005 by T-test for equality of means.
