Fig. 6: Hypothermia at 33 °C promoted Nrf2 nuclear translocation to inhibited ferroptosis. | Cell Death Discovery

Fig. 6: Hypothermia at 33 °C promoted Nrf2 nuclear translocation to inhibited ferroptosis.

From: Targeted temperature management alleviates post-resuscitation myocardial dysfunction by inhibiting ferroptosis

Fig. 6: Hypothermia at 33 °C promoted Nrf2 nuclear translocation to inhibited ferroptosis.The alt text for this image may have been generated using AI.

A Western blot of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 proteins in myocardial tissues. B–D Quantification of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 protein levels in myocardial tissues of each treatment group. (n = 6) E Western blot of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 proteins in myocardial tissues in cells. F–H Quantification of total Nrf2, cytoplasmic Nrf2, and nuclear Nrf2 protein levels in cells. (n = 6) I Immunofluorescence staining of Nrf2 in different treatment groups (DAPI staining shows nuclei). Scale bar:20 μm. J Quantification of Nrf2 immunofluorescence intensity in different treatment groups. (n = 6) K Immunoblot analysis of FPN1, FTH1, 4-HNE,Nuclear Nrf2 and NCOA4 under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation. β-actin and Histone H3 are used as loading controls. L–P Quantification of FPN1, 4-HNE,NCOA4,FTH1 and nuclear Nrf2 protein levels after H/R treatment and Nrf2 activator treatment. (n = 6) Q Oxidative stress markers:MDA levels, reflecting oxidative stress levels under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation.R Fluorescence microscopy images of Fe2+ under hypoxia/reoxygenation (H/R) conditions with or without Nrf2 activation. S Relative fluorescence intensity of Fe2+. Measurement data were presented as mean ± SD. Statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001.

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