Fig. 7: NFIX transcriptionally inhibited CDK1 through YBX1. | Cell Death Discovery

Fig. 7: NFIX transcriptionally inhibited CDK1 through YBX1.

From: NFIX suppresses breast cancer cell proliferation by delaying mitosis through downregulation of CDK1 expression

Fig. 7: NFIX transcriptionally inhibited CDK1 through YBX1.The alt text for this image may have been generated using AI.

A The CDK1 mRNA expression level after transfection with the NFIX expression plasmid. B The predicted binding sites of NFIX in the promoter region of CDK1. C The binding of NFIX to site1, site2, and site3 according to ChIP analysis. D Dual-luciferase reporter assays were used to analyze the regulation of CDK1 promoter activity by NFIX. E The top 10 interacting proteins identified by mass spectrometry. F The interaction between YBX1 and NFIX was verified using a co-IP assay. G The expression levels of CDK1 in NFIX-overexpressing cells with or without transfection with the YBX1 plasmid and in the corresponding control cells were determined by qRT-PCR. H Relationship between NFIX expression and YBX1 luciferase intensity. I The CDK1 expression level after transfection with the YBX1 expression plasmid. J The predicted binding sites of YBX1 in the promoter region of CDK1. K According to ChIP analysis, the binding of YBX1 to site1, site2, and site3 is in wild-type MCF7 cells. L Dual-luciferase reporter assays were used to analyze the regulation of CDK1 promoter activity by YBX1. M, N The binding of NFIX and YBX1 to the CDK1 promoter region after transfection of the NFIX plasmid was verified by ChIP analysis. O, P The binding of NFIX and YBX1 to the CDK1 promoter region after transfection of the YBX1 plasmid was verified by ChIP analysis. All experiments were repeated three times.

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