Fig. 1: Ex.50.T specifically targets GREM1.
From: Ex.50.T aptamer impairs tumor–stroma cross-talk in breast cancer by targeting gremlin-1
A Workflow of proteomic analysis and Venn diagrams of the number of proteins for each group. B Western blot of ex.50.T pull-down proteins. PDGFR-β was used as loading control for pulled-down protein; β-actin was used as the loading control of whole lysate; and GREM1 was detected with an anti-GREM1 antibody (Cell Signaling Technology, # 4383S). C ELONA for Ex.50.T on 30 nMol and 60 nMol of recombinant protein. D Increasing concentrations of ex.50.T or CtrlApt were incubated on plates uncoated or coated with GREM1 recombinant protein. Specific binding of ex.50.T was determined by subtracting the values obtained with CtrlApt and reported as Kd ± SE.
