Fig. 6: COL6A1 promoted the migration, invasion, and endothelial-like tube formation of HTR8/SVneo cells through APJ/AKT/mTOR pathway.

A, B The protein levels of COL6A1, p-AKT, AKT, p-mTOR, mTOR, and β-actin following COL6A1 knockdown or overexpression were measured by western blot. C–E COL6A1-overexpressed HTR8/SVneo cells and their corresponding control cells were treated with ML221 alone or combined with SC79. The protein levels of COL6A1, p-AKT, AKT, p-mTOR, mTOR, and β-actin were detected by western blot (C). Transwell assay (D) and endothelial-like tube formation assay (E) were performed (100×, scale bar: 100 μm).