Fig. 2: Effect of venetoclax on AML cell lines and WT1-CTLs. | Cell Death Discovery

Fig. 2: Effect of venetoclax on AML cell lines and WT1-CTLs.

From: BH3 mimetics augment cytotoxic T cell killing of acute myeloid leukemia via mitochondrial apoptotic mechanism

Fig. 2

A AML cell lines were incubated with venetoclax for 24 h in A221 media at the indicated concentrations (0, 10 nM, 50 nM, 100 nM, 500 nM, 1000 nM). Viability was assessed at 24 h using annexin V/PI staining. B WT1-CTLs from donors Hem07 (top), Hem14 (middle), or Hem15 (bottom) were first rested for 24 h in CTL media with IL-15 (5 ng/ml). After 24 h of resting, CTLs were transferred to CTL media without IL-15 (red lines) or with IL-15 5 ng/ml (blue lines) and with varying concentrations of venetoclax (0, 10 nM, 50 nM, 100 nM, 500 nM, 1000 nM). Viability was assessed after 24 h of venetoclax exposure using annexin V/PI staining. Statistical analyses were performed as a two-way ANOVA followed by post-hoc t-test with Bonferroni adjustment for multiple comparisons; ** = adjusted p < 0.01. Results are shown as mean ± standard deviation of n = 4 samples and are representative of 3 (A) or 2 (B) independent experiments.

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