Fig. 2: dCK knockdown promoted radiation-induced ferroptosis. | Cell Death Discovery

Fig. 2: dCK knockdown promoted radiation-induced ferroptosis.

From: HSP90 regulates dCK stability and inhibits ionizing radiation-induced ferroptosis in cervical cancer cells

Fig. 2

A Fe2+ concentrations in sh-dCK and sh-Control HeLa cells were found using the Phen GreenTMSK staining method. B–E Lipid peroxidation measurement in sh-Control and sh-dCK HeLa cells 48 h following 8 Gy of infrared radiation (B-C) and erastin or DMSO treatment (D, E). F Evaluation of lipid peroxidation in sh-Control and sh-dCK HeLa cells treated with 20 μM ferrostatin-1 or DMSO for 48 h prior to being exposed to an 8 Gy dose of IR. PTGS2 expression in sh-Control and sh-dCK HeLa cells was analyzed using qRT-PCR 48 h after the cells were exposed to 8 Gy of IR (G), erastin (H), and t-BHP (I). GPX4 expression in sh-Control and sh-dCK was examined using qRT-PCR. HeLa cells 48 h after being exposed to 8 Gy of IR (J), erastin (K), and t-BHP (L). The control groups received DMSO treatment for the same amount of time. The error bars represent means ± SD based on three independent repeats. * Denotes non-significance; **p < 0.01; ***p < 0.001. Using a two-tailed unpaired student’s t-test, P values were determined.

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