Fig. 9: Analysis of compound effects induced by pharmacological treatments in SW480 and D247MG cell lines after 72 h. | Cell Death Discovery

Fig. 9: Analysis of compound effects induced by pharmacological treatments in SW480 and D247MG cell lines after 72 h.

From: Comprehensive analysis of cellular metrics: From proliferation to mitochondrial membrane potential and cell death in a single sample

Fig. 9

A Multiparametric analysis of SW480 cells treated with 200 nM rotenone or 200 nM antimycin A for 72 h, compared to control cells. The bubble plot integrates changes in cell number (flow cytometry cell counting, y-axis), early apoptosis levels (annexin V/PI staining, bubble size), proliferation rate (CellTrace Violet staining, x-axis), and cell cycle dynamics (BrdU/PI staining, bubble color; values represent the sum of cells in the G1 and G2 phases). B Multiparametric analysis of D247MG cells treated with 20 μM temozolomide or 20 μM carmustine for 72 h, compared to control cells. The bubble plot integrates changes in cell number (flow cytometry cell counting, y-axis), number of cells with low mitochondrial membrane potential (MMP; JC-1 staining, bubble size), proliferation rate (CellTrace Violet staining, x-axis), and number of dead cells (annexin V/PI staining, bubble color). All bubbles represent means; n = 3.

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