Table 1 Stock reagents.
Reagent | Fluorochrome Ex/Em | Source | Catalog # | Weight | Volume | Stock [c] |
|---|---|---|---|---|---|---|
BrdU | - | Sigma-Aldrich St. Louis, Missouri, United States | 59-14-3 | 9.2 mg | 1.498 ml DMSO | 20 mM |
Alexa Fluor® 647 Mouse anti-BrdU antibody | 650/671 (filter for APC) | BD Franklin Lakes, New Jersey, United States | 560209 | – | – | – |
Propidium iodide | 535/617 (filter for PE) | Thermo Fisher Scientific Waltham, Massachusetts, United States | P3566 | – | – | 1 mg/ml (1.5 mM) |
CellTrace™ Violet | 405⁄450 (filter for BV421) | Thermo Fisher Scientific Waltham, Massachusetts, United States | C34557 | - | 50 μl DMSO | 2 mM |
JC-1 | 514/529 514/590 (filters for FITC and PE) | Enzo Life Sciences Farmingdale, New York, United States | ENZ-52304 | 5 mg | 1 ml DMSO | 7.66 mM |
Annexin V binding buffer | – | – | – | – | – | 10x |
APC Annexin V | 651/660 (filter for APC) | BD Franklin Lakes, New Jersey, United States | 550474 | – | – | – |
RNase A | – | Sigma-Aldrich St. Louis, Missouri, United States | R4875 | 10 mg | 1 ml PBS | 10 mg/ml |
Borax Anhydrous (Sodium teraborate) | – | Sigma-Aldrich St. Louis, Missouri, United States | 71997-100 G | 11.4 g | 300 ml ddH2O | 100 mM pH 8.5 |
Tween-20 | – | Sigma-Aldrich St. Louis, Missouri, United States | 8221841000 | – | – | 100% |
Triton X-100 | – | Sigma-Aldrich St. Louis, Missouri, United States | 1086031000 | – | – | 100% |
12-well Cell Culture Plate (or equivalent) | – | Fisher Scientific Hampton, New Hampshire, United States | 07-000-208 | – | – | – |
DMEM, high glucose, GlutaMAX™ Supplement, pyruvate | – | Thermo Fisher Scientific Waltham, Massachusetts, United States | 31966021 | – | – | – |
Trypsin-EDTA (0.25%), phenol red | – | Thermo Fisher Scientific Waltham, Massachusetts, United States | 25200072 | – | – | – |
