Fig. 1: Comparison of body weight changes, intratesticular cell composition, sperm malformations, and motility between DZN-treated and control mice. | Cell Death Discovery

Fig. 1: Comparison of body weight changes, intratesticular cell composition, sperm malformations, and motility between DZN-treated and control mice.

From: Diazinon induces testicular dysfunction and testicular cell damage through increased reactive oxygen species production in mouse

Fig. 1

A Body weight changes were measured weekly until the end of the experiment (0–11 weeks). Data are presented as the mean ± standard deviation (SD). B Comparison of testicular weight and seminiferous tubule diameter in DZN-injected and control mice. The data is shown as mean ± SD. C H&E staining of testes from DZN-injected and control mice, comparison of positive cells of DDX4 and SYCP3 in mouse testes of each experimental group. Scale bar = 100 μm. (D) Number of DDX4+ and SYCP3+ cells in testicular tubules. At least 40 tubules were scored for each testis (five to six biological replicates). Values are presented as the mean ± SD. (E) Protein expression levels of SYCP3 and DDX4 in testes of DZN-treated and control mice. Graph represents relative protein levels (mean ± SD, n = 5). (F) Expression levels of Sall4, Plzf, and piwii in testes from each group. Values are presented as the mean ± SD (log2 scale). (G) Expression levels of Sycp3, Dmc1, Rec8, Pgk2, and Tp1. Values on the graph are presented as the mean ± SD (log2 scale). (H) Comparison of sperm motility and parameter including distance average path (DAP, μm), distance curved line (DCL, μm), distance straight line (DSL, μm), velocity average path (VAP, μm/s), velocity curved line (VCL, μm/s), velocity straight line (VSL, μm/s) and percentage of fast motility, slow motility, local mortality and immortality through CASA analysis. (I) Microscopic analysis of sperm from each experiment group. The graph shows the proportion of abnormal structures in the head, tail, and neck of sperm from DZN-treated and control mice. Abnormality of sperm rations was calculated from seven independent experiments and measured 100 spermatozoa at least in each independent experiment. Asterisks indicate significant differences between the treatment and control groups at *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 50 μm. All statistical analyzes for the graphs were conducted using a t-test.

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