Fig. 1: Cellular localization of Satb2 in layer 6b.

a, b Colocalization of Satb2 with Ctgf and Cplx3 in layer 6b at P7. Satb2 (brown in the nucleus) was stained with an anti-Satb2 antibody, and Ctgf and Cplx3 (purple in the cytoplasm) were revealed by in situ hybridization. Cell counts show that the majority of Satb2⁺ neurons are co-labeled with Ctgf or Cplx3, and vice versa. c, d Colocalization of Satb2 with Trh and Tnmd in layer 6b at P7. Satb2 (brown in the nucleus) was stained with an anti-Satb2 antibody, and Trh and Tnmd (purple in the cytoplasm) were revealed by in situ hybridization. Cell counts show that Satb2⁺ neurons are present in these two subpopulations of layer 6b neurons. e, f Double immunostaining of Trh (red, e, f) with Ctgf (green, e) and Cplx3 (green, f) in layer 6b, showing that Trh represents a subpopulation of layer 6b neurons at P7. g Colocalization of BrdU and Satb2 in layer 6b of P7 mice with a single pulse of BrdU injection in pregnant mice at E11.5. Cell counts show that 40% of the Satb2⁺ neurons in layer 6b are born at this stage. h Dynamic expression of Satb2 protein and mRNA in the developing cortex at indicated timepoints. Both the protein and mRNA are clearly detected in the cortical plate including the subplate (Ctx) at E13.5, E14.5 and E17.5. Ctx cortex, sp subplate. Scale bars = 50 μm in (a–d), 25 μm in enlarged figures in (a–d), 20 μm in (e, f), 10 μm in enlarged figures in (e, f), 20 μm in (g), 10 μm in enlarged figure in (g), 20 μm in (h).