Fig. 3: Cellular localization of Nr4a2 in layer 6b.

a, b Colocalization of Nr4a2 with Cplx3 (a) and Trh (b) in layer 6b at P7. Nr4a2 (brown in nuclei) was revealed by immunohistochemistry, and Cplx3 and Trh (purple in cytoplasm) were revealed by in situ hybridization. Cell counts show that Nr4a2 is present in these two subpopulations of layer 6b neurons. Black arrowheads indicate double-labeled neurons and white arrowheads indicate single-labeled neurons. c Colocalization of BrdU (green) and Nr4a2 (red) in layer 6b of P7 mice with a single pulse of BrdU injection in pregnant mice at E11.5 and E12.5. Yellow arrowheads indicate double-labeled neurons and white arrowheads indicate single-labeled neurons. d Dynamic expression of Nr4a2 protein and mRNA in the developing cortex at indicated time points. Nr4a2 protein is clearly detected in the subplate at E15.5 and its mRNA is clearly detected in the subplate from E14.5. Black arrowheads indicate the subplate. Ctx cortex, sp subplate. Scale bars = 50 μm in (a, b), 20 μm in enlarged figures in (a, b), 50 μm in (c), 10 μm in enlarged figures in (c), 50 μm in (d).