Fig. 4: Altered gene expression in the layer 6b of Nr4a2^Emx1 CKO mice.

a The strategy for generating a mouse line with Cre-dependent Nr4a2 inactivation. b Loss of Nr4a2 expression in layer 6b of Nr4a2^Emx1 CKO mice compared with controls. c–i The cell number of Ctgf (c) and Cplx3 (d) is comparable between Nr4a2^Emx1 CKO and control mice, while the number of Trh⁺ (e) and Tnmd⁺ (f) neurons is increased in Nr4a2^Emx1 CKO mice compared with controls at P30. The cell number of Ctgf (g) and Cplx3 (h) is comparable between Nr4a2^Emx1 CKO and control mice, and the number of Trh⁺ (i) neurons is increased in Nr4a2^Emx1 CKO mice compared with controls at P7. j There is no change in the number of Satb2⁺ neurons or the ratio of Satb2⁺Ctgf⁺/Ctgf⁺ neurons between controls and Nr4a2^Emx1 CKO mice at P30. Ctx cortex. n = 4 for each group in c–j for statistics, *P < 0.05. Scale bars = 100 μm in (b), 100 μm in (c–i), 50 μm in inserts in (c–i), 50 μm in (j), 20 μm in inserts in (j).