Fig. 6: Cell autonomous role of Satb2 in the differentiation of layer 6b neurons.

a Satb2 is deleted in Nr4a2⁺ cells in layer 6b in Satb2^Nr4a2CreER CKO mice at P0 with tamoxifen injection at E15.5. b–d The expression of Ctgf (b), Cplx3 (c) or Trh (d) is significantly reduced in Satb2^Nr4a2CreER CKO mice compared to control mice at P0. The quantitation of Ctgf⁺, Cplx3⁺ and Trh⁺ cell counts is shown in (b–d). e Satb2 is deleted in Nr4a2⁺ cells in layer 6b in Satb2^Nr4a2CreER CKO mice with tamoxifen injection at P7. f–h The number of Ctgf⁺ (f), Cplx3⁺ (g) and Trh⁺ (h) cells is comparable between controls and Satb2^Nr4a2CreER CKO mice at P30. n = 3 for each group in a-h for statistics, *P < 0.05, **P < 0.01,***P < 0.001. Scale bars = 50 μm in (a), 20 μm in inserts in (a), 100 μm in (b, c), 20 μm in inserts in (b, c), 100 μm in (d), 20 μm in inserts in (d), 50 μm in (e), 20 μm in inserts in (e), 100 μm in (f–h), 50 μm in inserts in (f–h).