Fig. 4: Mitotic defects in p21-deficient cells following low-dose doxorubicin treatment. | Cell Death Discovery

Fig. 4: Mitotic defects in p21-deficient cells following low-dose doxorubicin treatment.

From: Dual role of p21 in regulating apoptosis and mitotic integrity in response to doxorubicin in colon cancer cells

Fig. 4

A Live-cell imaging of HCT116 p21−/− cells expressing GFP-H2B, treated with 100 nM doxorubicin for 48 h. Images were captured every 15 min over 9 h. Scale bar = 5 μm. B, C Immunofluorescence showing the localization of Aurora B (B) and MKLP1 (C) in WT, p53−/−, and p21−/− cells synchronized to early anaphase by a thymidine-RO-3306 block. Hoechst 33342 (blue), α-tubulin (red), and Aurora B or MKLP1 (green) are shown. Quantification of cells with weak Aurora B (B) and MKLP1 (C) signals in early anaphase (n = 25 cells per group, repeated 4 times). Data are mean ± SD, ****p < 0.0001. D, E Immunofluorescence showing Aurora B (D) and MKLP1 (E) localization in WT and p53−/− cells transduced with NT or p21 shRNA.

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