Fig. 3: UPS induction during OGD/R preserves mitochondrial functionality. | Cell Death Discovery

Fig. 3: UPS induction during OGD/R preserves mitochondrial functionality.

From: NRF1 coordinates mitochondrial adaptations to dampen intracellular ROS and inflammatory responses during ischemia reperfusion

Fig. 3: UPS induction during OGD/R preserves mitochondrial functionality.

A, B BMDMs were subject to different durations of OGD/R followed by analysis of poly-ubiquitinated proteins by WB(A). Ubiquitin protein levels were normalized to α-Tubulin (B). C BMDMs subject to OGD/R were analyzed for the expression of proteasome subunit genes by qRT-PCR. D-E. BMDMs subject to different durations of OGD/R were treated with MG132 (5 nM) in the last 1 h before harvest, followed by analysis of poly-ubiquitinated proteins by WB (D). Data are presented as fold induction of band intensity in the +MG132 condition versus the -MG132 condition for the corresponding OGD/R stimulation time point (E). F–H BMDMs subject to different durations of OGD/R were treated with MG132 during reoxygenation. DCFHA, MitoSox and TMRM staining was assessed by flow cytometry and MFI shown normalized to the untreated condition. Statistical test by one-way ANOVA and 2-tailed samples t-test (n = 3 and representative of 3 independent experiments, n.s. no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Back to article page