Fig. 1: MGP expression is increased in osteoporosis.

A H&E staining and IHC staining for MGP in the femurs of C57BL/6J mice subjected to sham surgery, OVX mice, young mice, and aged mice (n = 6). B, C The bone mineral density of nonosteoporotic (non-OP) patients and osteoporotic (OP) patients was measured via T scores, and the MGP expression levels in MSC lysates were analyzed via ELISA (n = 10). D The correlation between MGP expression and the T score is shown. E Immunofluorescence staining of MGP expression in bone marrow MSCs from non-OP and OP patients and the femurs of C57BL/6J mice subjected to sham surgery, OVX mice (n = 6). F The MGP expression levels in bone marrow MSCs from non-OP and OP patients and mice that underwent sham surgery and OVX were assessed through qRT‒PCR(n = 10). G The MGP protein levels in bone marrow MSCs from non-OP and OP patients and mice that underwent sham surgery and OVX were detected using Western blotting (n = 10). Scale bar = 50 μm. All the data are presented as the means ± SDs; Pearson correlation analysis was used to determine the correlation indices. Student’s t test or ANOVA was used to determine significant differences. ns not statistically significant; *P < 0.05; **P < 0.01; and ***P < 0.001.