Fig. 2: c-Cbl deficiency leads to motor dysfunction, reduced dopamine levels in the striatum, and loss of dopaminergic neurons in the substantia nigra.
From: The E3 ligase c-Cbl modulates microglial phenotypes and contributes to Parkinson’s disease pathology
A Representative movement trajectories during open field tests. B–D Changes in locomotor behavior in 3 month-old and 10 month-old c-Cbl-/- mice and wild-type (WT) littermates (n = 8–11): (B) total movement distance, (C) mean velocity, and (D) rest time were recorded in 30 min. E Changes in motor coordination and balance in 3 month-old and 10 month-old c-Cbl-/- mice and WT mice. F–H The levels of DA (F) and its metabolites, DOPAC (G) and HVA (H), in the striatum were analyzed by HPLC (n = 11-16). I The ratio of (DOPAC + HVA)/DA was analyzed. J Microphotographs of TH-positive neurons in the SNc of 3-month-old and 10 month-old c-Cbl-/- mice and WT mice. K Quantification of TH-positive neurons in the SNc (n = 6-8). Scale bars: 200 μm (above) or 50 μm (below). Data expressed as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 vs. age-matched WT. #p < 0.05, ##p < 0.01, ###p < 0.001 versus 3 month-old c-Cbl-/- mice.
