Fig. 4: Validation of the sumoylation of selected targets by SUMO2 under OGD and ROG. | Cell Death Discovery

Fig. 4: Validation of the sumoylation of selected targets by SUMO2 under OGD and ROG.

From: SUMO2/3 modification of transcription-associated proteins controls cell viability in response to oxygen and glucose deprivation-mediated stress

Fig. 4

Validation of proteomic results by selection of several proteins undergoing increased sumoylation under OGD and decreased sumoylation after ROG, as indicated by mass spectrometry, to test them by western blot with specific antibodies against the different proteins, before (extract) and after precipitation of His10-SUMO2 with His-bind Matrix (pull-down), in proliferating (A) and differentiated (B) cells. Twenty micrograms of total protein were loaded per lane in the case of extracts. α-TUBULIN (present in extracts but not after pull-down) was registered as a loading marker. Black arrowheads indicate unmodified proteins while white arrowheads indicate sumoylated products. C control conditions, O OGD, R ROG. *Unspecific band.

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