Fig. 3: CCNE1 sustains the stem-like properties of TNBC cells and fuels malignant progression through ANLN. | Cell Death Discovery

Fig. 3: CCNE1 sustains the stem-like properties of TNBC cells and fuels malignant progression through ANLN.

From: CCNE1 stabilizes ANLN by counteracting FZR1-mediated the ubiquitination modification to promotes triple negative breast cancer cell stemness and progression

Fig. 3

TNBCcells BT-549 and MDA-MB-231 with CCNE1 overexpression and ANLN knockdown were constructed. A The proliferative ability of TNBCcells was evaluated by MTT assay. B The migration of TNBCcells was tested by wound-healing assay. C WB assay was used to detect stemness markers protein expression in BT-549 and MDA-MB-231 cells. D The sphere-forming ability was detected in BT-549 and MDA-MB-231 cells. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001. E–G MDA-MB-231 cells with overexpression of CCNE1 and knockdown of ANLN were injected subcutaneously into mice to establish xenograft tumor model, and the growth and tumor size of mice were observed. H Typical images of immunohistochemistry staining of mice tumor tissue in overexpression of CCNE1 and knockdown of ANLN. I The protein expression levels of CCNE1, ANLN, and stemness markers in the tumor tissues was evaluated by WB analysis. n = 6, *P < 0.05, ***P < 0.001.

Back to article page