Fig. 3: Knockdown of endogenous MMP-10 ameliorates kidney injury and fibrosis in obstructive nephropathy.

A Experimental design. The red and green arrows show the timing of UUO surgery and plasmid injection, respectively. B, C Representative micrographs (B) and quantitative data (C) demonstrated MMP-10 protein expression in different groups as indicated. Scale bar, 50 µm. Data are presented as the mean ± SEM. **P < 0.01 versus sham controls (n = 5); ^†P < 0.05 versus UUO (n = 5). D, E Western blot analysis showed MMP-10 protein levels in various groups as indicated. Data are presented as the mean ± SEM. **P < 0.01 versus sham controls (n = 5), ^††P < 0.01 versus UUO (n = 5). F, G Western blot analysis showed the expression of several fibrosis-related proteins such as fibronectin, α-SMA, and vimentin. Representative Western blotting (F) and quantitative data (G) are presented. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 versus sham controls (n = 5); ^††P < 0.01, ^†††P < 0.001 versus UUO (n = 5). H, I Representative Western blotting (H) and quantitative data (I) showed KIM-1 and E-cadherin protein in various groups as indicated. Data are presented as the mean ± SEM. **P < 0.01 versus sham controls (n = 5), ^††P < 0.01 versus UUO (n = 5). J, K Representative micrographs (J) and quantitative data (K) showed immunohistochemical staining for α-SMA and KIM-1 and Sirius red staining for collagen deposition. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 versus sham controls (n = 5); ^†P < 0.05, ^††P < 0.01 versus UUO (n = 5). Scale bar, 50 µm.