Fig. 7: Inhibition of EGFR signaling blocks exogenous MMP-10-induced fibrotic lesions in UUO.
From: Matrix metalloproteinase-10 promotes kidney fibrosis by transactivating β-catenin signaling
A Immunohistochemical staining confirmed the co-localization of MMP-10, p-EGFR, and KIM-1 in serial sections of the obstructed kidney after UUO. Arrows indicate positive staining. Scale bar, 50 µm. B, C The expression levels of renal KIM-1 and MMP-7 in different groups were assessed by Western blotting. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 versus sham controls (n = 5); †P < 0.05, †††P < 0.001 versus UUO plus pcDNA3.1 group (n = 5); #P < 0.05, ###P < 0.001 versus UUO plus pFlag-MMP-10 group (n = 5). D, E Representative Western blotting (D) and quantitative data (E) showed renal expression of fibronectin and α-SMA in different groups. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 versus sham controls (n = 5); ††P < 0.01 versus UUO plus pcDNA3.1 group (n = 5); #P < 0.05, ##P < 0.01, ###P < 0.001 versus UUO plus pFlag-MMP-10 group (n = 5). F, G Representative micrographs (F) and quantitative data (G) showed renal expression of KIM-1 and fibronectin and fibrotic lesions by Sirius red staining. Scale bar, 50 µm. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 versus sham controls (n = 5); ††P < 0.01 versus UUO plus pcDNA3.1 group (n = 5); ##P < 0.01, ###P < 0.001 versus UUO plus pFlag-MMP-10 group (n = 5).
