Fig. 6: miR-139-5p promotes ferroptosis and inhibits cholesterol biosynthesis and tumour progression in vivo.

A Schematic diagram of animal experimental procedures. Created with www.figdraw.com. B 100 mg/kg MVA or 10 mg/kg SIM were injected intraperitoneally every three days to treat mice in the corresponding group, for five times. Bioluminescence imaging indicated the size of intracranial tumours under different treatments. The luminescent signal intensity of each group of glioma-bearing mice was statistically analysed. C LC–MS/MS was employed to quantify the levels of MVA in tumour tissues. D The total cholesterol content of the tumour tissues was detected using the cholesterol kit. These results align with in vitro experiments. E MDA kit was used to detect the content of MDA in tumour tissues. F The GSH content in tumour tissues was detected by GSH kit. G The results of the Balb/c nude mouse survival analysis were consistent with those of the in vitro experiments. Data are presented as mean ± standard deviation (SD) from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05.