Fig. 6: YAP-TEAD interaction inhibitor enhances the osteogenic differentiation of iPSC-EBs. | Cell Death Discovery

Fig. 6: YAP-TEAD interaction inhibitor enhances the osteogenic differentiation of iPSC-EBs.

From: Shaking culture attenuates circadian rhythms in induced pluripotent stem cells during osteogenic differentiation through the TEAD-Fbxl3-CRY axis

Fig. 6

a Real-time RT-PCR analysis of the mRNA levels of the osteogenic genes Runx2, Col1a1, and Ocn after 30 d of osteogenic induction. b Western blot analysis of the osteogenic marker OCN. c Quantitative analysis of OCN expression using ImageJ software. Expression levels were normalized to that of β-actin. d Western blot analysis of the osteogenic marker COL1A1. e Quantitative analysis of COL1A1 expression using ImageJ software. Expression levels were normalized to that of β-actin. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA and Tukey’s test; n = 3). f, g H&E and von Kossa staining of osteogenically induced iPSC-EB after 30 d of culture. Asterisks indicate osseous-like tissue regions and yellow arrows highlight calcified nodules. h, i Immunofluorescence staining analysis of OCN and COL1A1 in iPSC-EBs. Asterisks indicate osseous-like tissue regions, and white arrows mark positive cell staining for type I collagen and osteocalcin. Scale bars, 50 μm.

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