Fig. 1: β-catenin is critical for cell survival during the early phase of PS induction from hESCs. | Cell Death Discovery

Fig. 1: β-catenin is critical for cell survival during the early phase of PS induction from hESCs.

From: β-catenin safeguards cell survival via a transcription-independent mechanism during the induction of primitive streak from hESCs

Fig. 1

A The representative plots (left panels) and the summarized data (right panels) of flow cytometry for detection of cell death in wild-type (wt) cells during PS induction. Data are represented as mean ± SEM; n = 3; *p < 0.05, ***p < 0.001 vs. undifferentiated cells (0 h group); ##p < 0.01, ###p < 0.001 vs. the indicated groups; one-way ANOVA followed by Tukey’s post hoc. B Western blot analysis of β-catenin during early PS induction. Data are represented as mean ± SEM; n = 5; *p < 0.05, **p < 0.01 vs. undifferentiated cells (0 h group); one-way ANOVA followed by Tukey’s post hoc. C Immunofluorescence staining of β-catenin during early PS induction. Scale bar, 5 μm. D qRT-PCR analysis of gene expression during PS induction. (E) Cell morphology during PS induction. Scale bar, 100 μm. F Representative flow cytometry plots of dead cell. (G) The summarized data of dead cells in (F). Data are represented as mean ± SEM; n = 3; n.s., non-significant; *p < 0.05, **p < 0.01, ***p < 0.001 by comparing with the wt cells at the same time point; two-way ANOVA followed by Sidak’s post hoc. H Flow cytometry analysis of β-cat−/−-Ctrl and β-cat−/−-FL cells by Annexin V and PI co-staining after 8-h of PS induction. I The summarized data from (H). Data are represented as mean ± SEM; n = 3; ***p < 0.001; Student’s t test.

Back to article page