Fig. 5: β-catenin attenuates mitochondrial translocation of p53 and promotes mitophagy via inhibition of mTORC1 during PS induction. | Cell Death Discovery

Fig. 5: β-catenin attenuates mitochondrial translocation of p53 and promotes mitophagy via inhibition of mTORC1 during PS induction.

From: β-catenin safeguards cell survival via a transcription-independent mechanism during the induction of primitive streak from hESCs

Fig. 5

A Representative (left panel) and summarized data (right panels) of western blot analysis of p53 and LC3-II in mitochondria after 2 h of PS induction. ATPB, the mitochondria marker. PCNA, the nuclear marker. GAPDH, the cytoplasm marker. 0 and 2, the PS induction time (h); Rapa, rapamycin treated at 10 nmol/L; 2+Rapa, the cells treated with rapamycin for 2 h since the PS induction. Data are represented as mean ± SEM; n = 3; n.s., non-significant; **p < 0.01, ***p < 0.001; two-way ANOVA followed by Sidak’s post hoc. B MitoTracker Green (MTG) and LysoTracker Red (LTR) staining in wt and β-cat−/−-1 cells at 0 (undifferentiated stage) and 2 h after PS induction. C Representative (left panels) and summarized data (right panels) of Flow cytometry analysis of cell death in wt and β-cat−/−-1 cells with or without FCCP (1 μmol/L) treatment by Annexin V and PI co-staining. The cells are harvested after 8-h of PS induction. Data are represented as mean ± SEM; n = 3; **p < 0.01, ***p < 0.001; two-way ANOVA followed by Sidak’s post hoc. D The schematic of how mTORC1 inhibition promotes cell survival during PS induction.

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