Fig. 5: Cell death caspase - and Annexin 5 assays after TG and staurosporine treatment and representative TEM ultrastructure of RBL-1 cells, treated with staurosporine. | Cell Death Discovery

Fig. 5: Cell death caspase - and Annexin 5 assays after TG and staurosporine treatment and representative TEM ultrastructure of RBL-1 cells, treated with staurosporine.

From: Thapsigargin triggers a non-apoptotic, caspase-independent programmed cell death in basophilic leukaemia cells

Fig. 5

a Representative contour plot image of the caspase 3/7 assay FACS data for untreated cells. b Contour plot image of the 60 min TG caspase 3/7 assay FACS data, representative for all TG treatments. c Representative caspase 3/7 contour plot image for staurosporine. d Caspase 3/7 cell death assay shows on the X-axis untreated, 5 min-, 10 min-, 30 min, 60 min - and 180 min TG-treated RBL-1 cells as well as the caspase positive staurosporine control (180 min). The Y-axis reflects the caspase 3/7 activity, which is increased upon staurosporine treatment (20-26%). All other treatments as well as untreated cells show a caspase 3/7 activity below 3%. e Representative contour plot images of the Annexin V assay for untreated, 60 min TG treatment (f) and 180 min staurosporine-treated cells (g). h Annexin V assay shows on the X-axis untreated (0 min), 5 min-, 10 min-, 30 min, 60 min and 180 min TG-treated and staurosporine-treated RBL-1 cells. The Y-axis reflects the Annexin V activity, which is increased upon 30 min, 60 min and 180 min staurosporine treatment (6–15%). All other treatments as well as untreated cells show an Annexin V activity below 2%. i TEM overview of an RBL-1 cell after staurosporine treatment (180 min), showing apoptotic features such as nuclear fragmentation and chromatin condensation (n) as well as swollen ER (er) and apoptotic bodies (arrows).

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