Fig. 2: Functional experiments were performed to elucidate the involvement of histone lactylation in the malignant biological behavior of PC cells.

A, B The expression levels of Pan Kla, H3K18la, Bax and Bcl2 in PANC-1 and ASPC-1 cells after LDHA silencing were detected by western blot. Colony formation (C) transwell invasion (D) CCK-8 (E) wound-healing (F) and EdU (G) assays were used to explore the effects of H3K18la inhibition on the proliferation, migration and invasion of PC cells. All data are shown as the mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, vs. NC. #p < 0.05, ##p < 0.01, ###p < 0.001, vs. si-LDHA.