Fig. 1: The impact of SCI on microglial polarization and USP29 expression in spinal cord tissues. | Cell Death Discovery

Fig. 1: The impact of SCI on microglial polarization and USP29 expression in spinal cord tissues.

From: ELAVL1-mediated USP29 mRNA degradation activates TAK1 driving M1 microglial polarization and neural stem cell differentiation dysregulation in spinal cord injury

Fig. 1

A Schematic diagram of the SCI rat model construction. B BBB scores of rats in each group at days 1, 3, 7, 14, 21, and 28 post-SCI for motor function assessment. C Inclined plane test scores of rats in each group at days 1, 3, 7, 14, 21, and 28 post-SCI, used to assess motor coordination. D Representative images of HE staining of spinal cord tissue on 28 d in each group (scale bars: 1000 μm and 200 μm). E Representative images of Nissl staining of spinal cord tissue on 28 d in each group (scale bars: 1000 μm and 200 μm) and the quantification of surviving neurons. F Representative images of Iba1 and CD80 IF staining on 7 d (scale bar = 100 μm), and quantification of average fluorescence intensity of Iba1+ cells, CD80+ cells and related fluorescence intensity ratio. G Western blot analysis and protein quantification of USP29 expression in spinal cord tissue of each group on 7 d. H Representative images of USP29 IHC staining in spinal cord tissue on 7 d (scale bars: 1000 μm and 200 μm), and quantification of the percentage of USP29-positive cells. I Representative images of Iba1 and USP29 IF staining on 7 d (scale bar = 100 μm) as well as quantification of USP29 +Iba1+/Iba1+ fluorescence intensity ratio. N = 6, *** indicates p < 0.001, **** indicates p < 0.0001.

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