Fig. 5: Effects of ELAVL1 on USP29 mRNA. | Cell Death Discovery

Fig. 5: Effects of ELAVL1 on USP29 mRNA.

From: ELAVL1-mediated USP29 mRNA degradation activates TAK1 driving M1 microglial polarization and neural stem cell differentiation dysregulation in spinal cord injury

Fig. 5

A Prediction of the RNA-binding protein (RBP) that binds to USP29 mRNA through the ENCORI database. B Western blot analysis of ELAVL1 protein expression in spinal cord tissues of rats (N = 6) on 7 d. C Western blot analysis of ELAVL1 protein expression in cells of each group. D RT-qPCR analysis of ELAVL1 expression in cells of each group. E RT-qPCR analysis of ELAVL1 and USP29 mRNA expression in cells of each group. F Western blot analysis of ELAVL1 and USP29 protein expression in cells of each group. G RT-qPCR analysis of USP29 mRNA expression in cells at different time points (0, 1, 2, 3 h) after treatment with Actinomycin D. H RIP-qPCR analysis of the interaction between ELAVL1 and USP29 mRNA, with Western blot validation of the RIP experiment and RT-qPCR detection of the level of USP29 mRNA enriched by ELAVL1. ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All cell experiments were repeated three times.

Back to article page