Fig. 7: Impact of HMC3 cells polarization on the fate differentiation of HNSCs. | Cell Death Discovery

Fig. 7: Impact of HMC3 cells polarization on the fate differentiation of HNSCs.

From: ELAVL1-mediated USP29 mRNA degradation activates TAK1 driving M1 microglial polarization and neural stem cell differentiation dysregulation in spinal cord injury

Fig. 7

A Representative IF images of Nestin and TUJ1, and Nestin and GFAP co-staining in spinal cord tissue on 7 d (scale bar = 100 μm), and quantification of the average fluorescence intensity of Nestin+ cells as well as the TUJ1+/Nestin+ or GFAP+/Nestin+ fluorescence intensity ratio, N = 6. B Diagram showing the co-culture of HMC3 cells and HNSCs. C EdU staining to detect HNSCs proliferation in each group, scale bar = 200 μm. D Representative IF images of TUJ1 and GFAP in HNSCs from each group (scale bar = 100 μm), and quantification of the average fluorescence intensity of TUJ1 and GFAP. E RT-qPCR measurement of TUJ1 and GFAP mRNA expression in HNSCs from each group. F EdU staining to detect HNSCs proliferation in each group (scale bar = 200 μm). G Representative IF images of TUJ1 and GFAP in HNSCs from each group (scale bar = 100 μm), and quantification of the average fluorescence intensity of TUJ1 and GFAP. H RT-qPCR of TUJ1 and GFAP mRNA expression in HNSCs from each group. All cell experiments were repeated 3 times, ns indicates p > 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001.

Back to article page