Fig. 1: Assessment of hAMSC viability after 3, 24 and 48 h of exposure to increasing BPA concentrations. | Cell Death Discovery

Fig. 1: Assessment of hAMSC viability after 3, 24 and 48 h of exposure to increasing BPA concentrations.

From: Bisphenol-A disrupts mitochondrial functionality leading to senescence and apoptosis in human amniotic mesenchymal stromal cells

Fig. 1

Morphological differences in hAMSC after 24 h of exposure with increasing concentrations of BPA (0.05, 0.1, 0.2, 0.3, 0.35, and 0.4 μM) (A). Images were acquired at ×20 magnification; scale bar, 100 μm. B Quantification of viable cells following 3, 24, and 48 h of BPA exposure, assessed via eFLUOR-positive staining. C Mitochondrial activity in hAMSCs after 3, 24, and 48 h of exposure, evaluated using MTT and ATPlite assays. D Absolute cell counts obtained by flow cytometry corroborate previously reported findings. Data are presented as mean ± SD from at least three independent experiments (n ≥ 3). Statistical significance was determined relative to the control condition (MetOH): **p < 0.01, ***p < 0.001, ****p < 0.0001.

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